Regulation of Corticosteroidogenic Genes by MicroRNAs

Figure 3

Results from Dicer1 siRNA transfection of H295R adrenocortical cells. Cells were transfected with Dicer1 siRNA or with a scrambled negative-control siRNA. Cortisol-related steroid levels for 11-deoxycortisol (a), cortisol (b), and cortisone (c) and aldosterone-related steroid levels for deoxycorticosterone (d), corticosterone (e), 18-OH-corticosterone (f), and aldosterone (g) were measured 48 hours posttransfection by LC MS:MS. Results represent the mean of three independent biological experiments performed in triplicate ± SEM; , , and compared to scrambled control.

(a)

(b)

(c)

(d)

(e)

(f)

(g)