Prolactin and Male Fertility: The Long and Short Feedback Regulation
Figure 8
Western blots of protamine 1 in epididymal sperm of
sexually mature rats depicting the presence of 6 Kd protamine 1 bands in
control and absence in drug-treated rats. F: Fluphenazine; C: Control; N: negative
control. Basic proteins were extracted in HCl, differentially extracted in
trichloroacetic acid, analysed on 15% acid urea PAGE. Western blotting was done with
1Hup 1N monoclonal antibody provided by Dr Rod Balhorn [61].