The Effect of Differentiation of Human Dental Pulp Stem Cells to Glial Cells on the Sensory Nerves of the Dental Pulp

Figure 4

Isolation and identification of trigeminal nerve: (a-i) the skull was cut along the left and right sides, exposing the area between the eyes, and then the bone was lifted. Subsequently, the brain was removed. (a-ii) The base of the skull is exposed. (a-iii) This panel illustrates the structure and composition of the trigeminal nerve. (a-iv) Ornithine encapsulation was performed overnight, followed by the addition of a laminin coating. (a-v) The images depict trigeminal neurons at different culture stages. (b) Immunofluorescent staining reveals MAP2, TUBB3, and NF200 expression in trigeminal axons. Scale bar: 20 μm. NF-200, neurofilament 200; TUBB3, β-III tubulin; and MAP2, microtubule-associated protein 2.