Polymodal Activation and Desensitization of TRPV1 Receptor in Human Odontoblasts-Like Cells with Eugenol

Figure 4

Immunocytochemical detection of TRPV1 receptor in hOLCs. Immunocytochemical identification of TRPV1 in the control of urethral epithelial tissue, in dental tissue, in hDPSCs cells, and in hOLCs. Anti-TRPV1 antibody conjugated with Alexa fluor 594 (red) was used to obtain the protein labeling and the nuclei were counterstained with Hoechst stain (blue). Note the positive staining in the dental tissue located in the area of the odontoblast palisade. Additionally, in hOLCs cells, an increase in this immunostaining localized in the membrane is evident, compared to hDPSCs. Zeiss Axio Imager A2 microscope was used. Scale bar: 50 µm.