Association between IFN-γ +874A/T and IFN-γR1 (-611A/G, +189T/G, and +95C/T) Gene Polymorphisms and Chronic Periodontitis in a Sample of Iranian Population

Figure 1

Electrophoresis pattern of ARMS-PCR for detection of IFN-γ (+874A/T) (a) and RFLP-PCR IFN-γR1 (-611A/G), (+189T/G), and (+95C/T) gene polymorphism (resp., (b), (c), and (d)). (a) Lines 1 and 8: marker 100 bp; Lines 2 and 3: product sizes were 264 bp for forward T allele primer (FT) and reverse primer (R); Homozygote TT; Lines 6 and 7: product sizes were 264 bp for forward A allele primer (FA) and reverse primer (R); Homozygote AA; Lines 4 and 5: product sizes were 264 bp for FT, FA, and R; Heterozygote AT; (b) Lines 1 and 8: marker 100 bp; Lines 2 and 5: Homozygote AA; Lines 4 and 7: Homozygote GG; Lines 3 and 6: Heterozygote AG; (c) Lines 1 and 8: marker 100 bp; Lines 2 and 5: Homozygote TT; Lines 4 and 7: Homozygote GG; Lines 3 and 6: Heterozygote GT; (d) Lines 1 and 10: Marker 100 bp, Lines 2, 5, and 8: Homozygote CC; Lines 4 and 7: Homozygote TT; Lines 3, 6, and 9: Heterozygote CT.

(a)

(b)

(c)

(d)