Structure of a Rhamnogalacturonan Fragment from Apple Pectin: Implications for Pectin Architecture

Figure 1

Evaporative light scattering profile of LW on anion-exchanged chromatography. The major components in each peak 2–21 were identified as oligomers with the following molecular formulas by MALDI-TOF MS: (1) not identified; (2) Rha₂GalA₂Gal₄Ara; (3) Rha₂GalA₂Gal₄; (4) Rha₂GalA₂Gal₃, Rha₂GalA₂Gal₂; (5) Rha₂GalA₂Gal₂; (6) Rha₂GalA₂GalAra; (7) Rha₂GalA₂Gal; (8) Rha₂GalA₂; (9) Rha₃GalA₂Gal₂, Rha₃GalA₃Gal₂, Rha₂GalA₃GalAra; (10) Rha₃GalA₃Gal, Rha₃GalA₃GalAra; (11) Rha₃GalA₃Gal, Rha₃GalA₃, Rha₃GalA₂AcetylGalAGal; (12) Rha₃GalA₃, Rha₃GalA₂AcetylGalAGal, Rha₃GalAAcetylGalA₂; (13) Rha₃GalA₂AcetylGalA, Rha₃GalA₃, Rha₄GalA₃AcetylGalA, Rha₄GalA₄; (14) Rha₂GalA₃, Rha₄GalA₃AcetylGalA; (15) Rha₄GalA₃AcetylGalA, Rha₂GalA₃; (16) Rha₄GalA₃AcetylGalA, Rha₄GalA₂AcetylGalA₂, Rha₂GalA₃; (17) Rha₃GalA₄, Rha₂AcetylGalA₄; (18) Rha₂AcetylGalA₄, Rha₃GalA₄, Rha₂GalA₄MethylGalA; (19) Rha₂GalA₄MethylGalA; (20) GalA₂; (21) GalA₃. The order of fragments in each peak is given according to their intensity in the MALDI-TOF MS spectrum.