Isolation of Female Germline Stem Cells from Mouse and Human Ovaries by Differential Adhesion

Figure 4

Characteristics of human female germline stem cells. (a) Phase contrast images of human ovarian cells after the isolation (A) and culture on different days (C and D); viability percentage of human ovarian cells after the isolation by using PI staining analyzed by BD FACS Aria II. (b) Immunofluorescence staining for germline markers in fGSCs on day 20 of culture in MEF-coated plates. (c) OLCs formed spontaneously from the fGSCs in the culture. Phase contrast and immunofluorescence staining images for oocyte markers (GDF9, ZP1, and VAZA) of differentiated OLCs in the culture.

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