Stellettin B Isolated from Stelletta Sp. Reduces Migration and Invasion of Hepatocellular Carcinoma Cells through Reducing Activation of the MAPKs and FAK/PI3K/AKT/mTOR Signaling Pathways

Figure 4

Stellettin B reduced the expressions of MMP-2, MMP-9, and uPA, and increased the expressions of TIMP-1 and TIMP-2. HA22T and HepG2 cells were treated with different concentrations of Stellettin B (0, 6, 12,18, 24 μM) for 24 h, and conditioned media and cell lysates were collected for analysis. (a) Examination of MMP-2/-9 activities by gelatin zymography. (b) The expression levels of MMP-2/-9, uPA, and TIMP-1/-2 were analyzed by western blotting of total cell lysates of HA22T and HepG2 cells treated with Stellettin B. Zero refers to cells treated with DMSO vehicle as a control. β-Actin was used as the internal control.

(a)

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