Morphology of Mitochondria in Syncytial Annelid Female Germ-Line Cyst Visualized by Serial Block-Face SEM

Figure 2

Organization of the mitochondria in syncytial germ-line cysts. (a) and (b) The accumulations of mitochondria (stars) are located mainly in the one pole of the nurse cells (NC) opposite the ring canal (bilateral dotted arrow in (b)), while in the cytophore (Cy) the mitochondria were evenly scattered in the cytoplasm. Nuclei of the nurse cells and oocyte (O) are marked as Nu; tubules of the endoplasmic reticulum as ER. (c) and (d) the mitochondria (stained black) are visible passing through the ring canals (arrows) connecting the nurse cells (NC) to the cytophore (Cy). Nu – nurse cell nucleus. (a, c, d) Light microscopy, Epon semi-thin sections stained with methylene blue. (a) Bar = 73 µm; (c, d) bar = 30 µm. (b) Transmission electron microscopy, Epon ultrathin section; bar = 2 µm.