Inhibition of TGF-β1 Signaling by IL-15: A Novel Role for IL-15 in the Control of Renal Epithelial-Mesenchymal Transition: IL-15 Counteracts TGF-β1-Induced EMT in Renal Fibrosis
Figure 6
Inhibition of rhTGF-β1-induced Snail1 expression by rhIL-15 involved the activation of C-jun pathway. (a) C-Jun phosphorylation and Snail1 expression were analyzed by western blotting after a 6h rhTGF-β1 treatment (3 ng/mL) in HK-2 cells pretreated or not with rhIL-15 for 30 min or 24h of (1 ng/mL). Bar charts represent phospho-c-Jun and Snail1 expressions normalized to GAPDH and β-actin, respectively. (p<0.05, n=3, ±SEMs). Snail1 expression analyzed by fluorescent immunostaining (b) or western blotting (c) after a 6h rhTGF-β1 (3 ng/mL) treatment in HK-2 cells pretreated or not for 24h with rhIL-15 (1 ng/mL). In some conditions, the specific JNK inhibitor SP600125 was added. To visualize cells, cell nuclei were stained with DAPI (insets). Original magnification ×25. Immunofluorescence data are representative of three independent experiments. Bar chart represents Snail1 expression normalized to β-actin, respectively (p<0.05, n=3, ±SEMs).