Nesprin-2 Interacts with Condensin Component SMC2

Figure 5

SMC2 (a) and SMC4 (b) in HaCaT keratinocytes treated with control shRNA (upper panels) and treated with Nesprin-2-SMC domain specific shRNA (lower panels). Nesprin-2 was detected with mAb K20-478. Bar, 10 μm. (c) Localization of Nesprin-2 after siRNA mediated knockdown of SMC2 in COS7 cells. Staining was with SMC2 specific antibodies and mAb K20-478 for Nesprin-2. Bar, 5 μm. (d) Evaluation of the SMC2 knockdown. SMC2 fluorescence intensity was measured in the center of mitotic chromosomes. 10 siRNA treated cells and 12 control cells (control treatment) were analyzed ( value = 0.0001).

(a)

(b)

(c)

(d)