Carbohydrate Moieties and Cytoenzymatic Characterization of Hemocytes in Whiteleg Shrimp Litopenaeus vannamei
Table 1
Lectins used to investigate cell surface oligosaccharides domains in hemocytes and their competitive sugars used as controls for lectin-binding.
Lectin
Latin name
Source
Resuspension solution
Specificity sugar
Inhibition
Con-A
Canavalia ensiformis (Sigma, C7642)
Jack bean
NaCl 0.9% pH 6.5 with 5 mM CaCl2
a-D-Mannose, a-D-glucose
Glucose
WGA
Triticum vulgaris (Sigma, L4895)
Wheat germ
PBS pH 7.4
(D-GlcNAc), NeuNAc
N-Acetylglucosamine
PNA
Arachis hypogaea (Sigma, L7381)
Peanut
PBS pH 7.4
-Galactose(1-3) galNAc
Galactosamine
UEA-I
Ulex europaeus (Sigma, L9006)
Gorse, furze
NaCl 0.9%
a-L-Fucose
Fucose
LEA
Lycopersicon esculentum (Sigma, L0401)
Tomato
(glcNAc)3
N-Acetylglucosamine
GalNAc = N-acetyl-galactosamine, GlcNAc = N-acetyl-glucosamine, and NeuNAc = N-acetylneuraminic acid. Competing sugar used in the study at 0.2 M. 10 mM HEPES, 0.15 M NaCl, 0.1 mM Ca2+, 0.08% sodium azide, and 5 mg mL−1β-cyclodextrin.