Research Article

Measured Effects of Wnt3a on Proliferation of HEK293T Cells Depend on the Applied Assay

Figure 1

Western blot analysis of HEK293T cells treated with 0–200 ng/mL Wnt3a for 24, 48, or 72 h. β-catenin slightly increased 24 hours after treatment. β-actin antibody was used as loading control. For quantification, the protein levels were normalized to the level of β-actin and the untreated control was set to 1.