Correlative Light and Electron Microscopy Reveals the HAS3-Induced Dorsal Plasma Membrane Ruffles

Figure 4

Ultrastructure of GFP-HAS3-positive dorsal ruffles. 2D confocal images showing the dorsal GFP-HAS3 signal are shown in (a) and (d) and corresponding SEM images in (b) and (e). Merged images are shown in (c) and (f), respectively. Many of the dorsal ruffles were linear or curved in shape (a) and provided a basis for several thinner protrusions (arrows in (b)). Occasionally, circular ruffles were detected (arrows in (d)-(e)). Scale bar 2 μm.

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