Correlative Light and Electron Microscopy Reveals the HAS3-Induced Dorsal Plasma Membrane Ruffles

Figure 2

GFP-HAS3-induced plasma membrane protrusions imaged by CLEM. A GFP-HAS3 expressing MCF-7 cell imaged by 3D confocal microscopy in (a), (d), and (g) and by SEM in (b), (e), and (h). Merged images are presented in (c), (f), and (i). High magnification images from selected areas of the same cell presented in (a)–(c) are shown in (d)–(i). Arrows in (d), (e), (g), and (h) indicate the GFP-HAS3-positive bulbous expansions in both the body and tips of protrusions. Color depth coding was used to demonstrate the variable length of protrusions in (d). Scale bars 10 μm in (a), 2 μm in (d) and (g).

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