HARE-Mediated Endocytosis of Hyaluronan and Heparin Is Targeted by Different Subsets of Three Endocytic Motifs

Figure 5

Endocytosis of HA and Hep by HARE CD variants is blocked by hyperosmolar conditions. Cells expressing EV, WT, or the indicated single-motif deletion HARE CD mutants were grown and pretreated as in Figure 2 and then preincubated at 37°C for 30 min with Endocytosis Medium with (white) or without (black) 0.45 M sucrose. The cells were then incubated with ¹²⁵I-labelled HA (a) or Hep (b) at 37°C for 4 h and processed as described in Methods section. Values are means ± SE () and significant differences (assessed by Student’s -test) between control and sucrose-treated samples are indicated: ^#; ; .

(a)

(b)