Research Article
Effect of Serum and Oxygen Concentration on Gene Expression and Secretion of Paracrine Factors by Mesenchymal Stem Cells
Table 3
Normalized relative expression of mRNA for paracrine factors.
| | Normoxia | Hypoxia | | 5% | 20% | 5% | 20% |
| VEGF () | 0.535 ± 0.038a | 0.667 ± 0.067 | 0.725 ± 0.051b | 0.812 ± 0.085 | MCP-1 () | 1.162 ± 0.145 | 1.410 ± 0.231 | 0.259 ± 0.063b | 0.557 ± 0.214b | MIP-1α () | 0.688 ± 0.042 | 0.757 ± 0.051 | 1.196 ± 0.136ab | 1.578 ± 0.061b | MIP-1β () | 0.690 ± 0.069 | 0.600 ± 0.036 | 0.932 ± 0.101ab | 1.290 ± 0.127b | MMP-2 () | 0.855 ± 0.039a | 0.583 ± 0.064 | 0.886 ± 0.066 | 0.718 ± 0.135 |
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Data reported as mean ± SE. 5% and 20% refer to the amount of serum supplement present in the media. versus 20%; versus normoxia.
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