Pharmacological Characterization of the Mechanisms Involved in Delayed Calcium Deregulation in SH-SY5Y Cells Challenged with Methadone
Figure 2
Methadone alters mitochondrial morphology. Morphology of methadone-treated cells was studied by confocal imaging of SH-SY5Y cells transfected with the pDsRed2-mito vector. Twenty-four hours after transfection, cell cultures were incubated for 3 h in the absence or presence of 0.25 or 0.5 mM methadone. Shown are images of the representative mitochondrial morphology in non-treated cells (control, (a)) or cells treated with methadone ((b), 0.25 mM; (c), 0.5 mM). Scale bar indicates 10 m. (d), The fractions of cells with filamentous, intermediate or punctuate mitochondrial patterns were determined in at least 6 independent cultures. (; ; Student's t-test versus basal conditions as indicated.)
