Effects of Substrate Mechanics on Contractility of Cardiomyocytes Generated from Human Pluripotent Stem Cells
Figure 6
Contraction stress remained stable with time past differentiation, while beating rate changed on soft substrates. H9-derived cardiomyocytes were seeded onto polyacrylamide hydrogels at 30 and 60 days postdifferentiation and imaged 24 hours later to obtain contraction stress and beating rate. (a) Average contraction stress increased with substrate stiffness but was not significantly different on any stiffness at D30 and D60 (overall for effect of time via two-way ANOVA). (b) Maximum contraction stress increased with substrate stiffness but was not significantly different on any stiffness at D30 and D60 (overall for effect of time via two-way ANOVA). For (a) and (b), -9 cells per stiffness for D30 and 9–13 cells per stiffness for D60. (c) Stiffness did not significantly affect beating rates of D60 H9-derived cardiomyocytes (overall via one-way ANOVA), but beating rate increased on soft substrates between D30 and D60. *() indicates statistically significant differences. cells per stiffness for D30 and 10–13 cells per stiffness for D60. For (a)–(c), error bars represent SEM.
