Differentiation Potential of Pancreatic Fibroblastoid Cells/Stellate Cells: Effects of Peroxisome Proliferator-Activated Receptor Gamma Ligands
Figure 6
Analysis of collagen synthesis. (a) Procollagen I propeptide (PIP) secretion of hPFC presented as percent of control after 24, 48, and 72 h of ciglitazone (3.3 μM) and PGJ2 (5.0 μM) treatment. The reduction of PIP content in culture supernatants was significant, except for 48 h PGJ2 (#, n ≥ 3). (b) An example of PIP as ng/mg protein for an individual isolate, representing an individual response towards treatment, and changes in cellular collagen synthesis over time in culture. (c) Depiction of collagen synthesis in control cells of the individual isolates used in this analysis as mean, minimum, and maximum values (n ≥ 3). (d) Collagen synthesis in transformed cells (HPF-T) after 24 h of 3.3 μM ciglitazone and 5.0 μM PGJ2 treatment expressed as percent of control. Within the first 24 h, HPF-T cells responded to ciglitazone by a significant induction of collagen synthesis, while the natural ligand inhibited collagen synthesis significantly (n ≥ 3).
