Differentiation Potential of Pancreatic Fibroblastoid Cells/Stellate Cells: Effects of Peroxisome Proliferator-Activated Receptor Gamma Ligands

Figure 5

PCR Analysis of lipid metabolism proteins. PCR analysis was carried out on two individual isolates of hPFC cells under control conditions (con) and after 24 h, 48 h, and 72 h ciglitazone treatment at 3.3 μM ciglitazone for HPF55ECM cells, and control and 72 h ciglitazone at 3.3 μM for HPF53RTC cells. For normalisation, β-actin was amplified. Fatty acid binding protein (FABP) showed a steady rise in expression over 24–72 h being highest at 72 h. Lipoprotein lipase was induced after 72 h of ciglitazone treatment though mRNA levels showed high variations, even under control conditions between isolates. PPARγ was induced at mRNA level after 24 h of treatment and highest after 72 h for both samples tested. PCR analysis shows the differences between individual isolates of hPFC, while the general responsiveness towards ciglitazone treatment was similar, basal expression and induction values varied.