Differentiation Potential of Pancreatic Fibroblastoid Cells/Stellate Cells: Effects of Peroxisome Proliferator-Activated Receptor Gamma Ligands
Figure 4
Cell cycle analysis. (a) Cell cycle analysis in hPFC cells after 24 h, 48, and 72 h of treatment with 3.3 μM ciglitazone. The rise in G1 phase cells (black) was significant after 24 h (*) and 72 h (*) compared to concomitant control values. S phase is depicted in hatched columns, and G2/M phase is depicted in grey, n ≥ 3. (b) Cell cycle analysis in PFC1 cells shows a steady rise in G1 phase cells over the time of culture, no matter if treated or not. At 72 h of ciglitazone treatment, the difference compared to controls was significant (*), as well as compared to 48 h of treatment (n ≥ 3). Vinblastine- (VB-) treatment showed a significantly reduced number of G1 cells compared to 72 h untreated controls (°°) and 72 h ciglitazone treatment (°), and a high level of G2/M phase cells. These effects were diminished by ciglitazone: G1 phase cell numbers were not significantly different from 72 h ciglitazone only (#), and G2/M phase accumulation was significantly reduced (+).
