Differentiation Potential of Pancreatic Fibroblastoid Cells/Stellate Cells: Effects of Peroxisome Proliferator-Activated Receptor Gamma Ligands

Figure 2

Lipid content evaluated by nile red binding. The figure shows the effect of 72 h of ciglitazone treatment on human pancreatic fibroblastoid cells (hPFCs, hatched columns), transformed hPFCs (HPF-T grey columns), and rat PFC1 cells (black columns) in comparison. Nile red binding to cells was evaluated by flow cytometry and calculated as % of nile red-positive cells. Ciglitazone-induced nile red binding in all cells tested, and hPFC showed a high variation of lipid content in untreated cells with the mean higher than any other cell line tested, but with P = 0.053, the rise in lipid content was not significant. (Depicted are means plus standard deviation, * significant versus untreated, n ≥ 3.)