Designing a Long Acting Erythropoietin by Fusing Three Carboxyl-Terminal Peptides of Human Chorionic Gonadotropin Subunit to the N-Terminal and C-Terminal Coding Sequence
Figure 3
Expression of EPO-WT and EPO-(CTP)3 from transfected CHO cells. Conditioned media from transfected cells were prepared for SDS/PAGE and proteins were detected by Western blot as described under “Materials and Methods.”