Band 3 Missense Mutations and Stomatocytosis: Insight into the Molecular Mechanism Responsible for Monovalent Cation Leak
Figure 2
Dose response curves of Cl−/ exchange by DIDS. The capacity of oocytes expressing wt AE1 or R760Q mutant (10 ng cRNA coinjected with 2.5 ng GPA-cRNA in both cases) to alkalinize in CO2/ buffer without extracellular Cl− (gluconate medium) was assessed in presence of different DIDS concentrations. The initial slope of alkalinization as a function of time was plotted against DIDS concentrations. The method used was described in a previous publication [25]. Data are means +/− s.e.m. of 9 to 20 oocytes from different batches. Black symbols correspond to wt AE1 expressing oocytes, empty circles to R760Q mutant expressing oocytes.
