Research Article
Characterization and Potential Application of Bromelain from Pineapple (Ananas comosus) Waste (Peel) in Recovery of Silver from X-Ray Films
Table 2
pH stability of the enzyme at different preincubation periods.
| pH value | Preincubation period | 0 h | 3 h | 6 h | 12 h | 24 h | 48 h | Gelatin removal time (seconds) | Residual activity (100%) | Gelatin removal time (seconds) | Residual activity (%) | Gelatin removal time (seconds) | Residual activity (%) | Gelatin removal time (seconds) | Residual activity (%) | Gelatin removal time (seconds) | Residual activity (%) | Gelatin removal time (seconds) | Residual activity (%) |
| 3 | 370 | 100 | 410 | 90.24 | 492 | 75.20 | 630 | 58.73 | 827 | 44.74 | 1230 | 30.08 | 4 | 49 | 100 | 55 | 89.09 | 65 | 75.39 | 81 | 60.49 | 129 | 37.98 | 250 | 19.60 | 5 | 44 | 100 | 45 | 97.78 | 46 | 95.65 | 45 | 97.78 | 46 | 95.65 | 80 | 55.00 | 6 | 38 | 100 | 39 | 97.44 | 41 | 92.68 | 42 | 90.48 | 45 | 84.44 | 51 | 74.51 | 7 | 34 | 100 | 34 | 100 | 36 | 94.44 | 35 | 97.14 | 36 | 94.44 | 39 | 87.18 | 8 | 45 | 100 | 47 | 95.74 | 50 | 90.00 | 55 | 81.81 | 60 | 75.00 | 480 | 9.38 | 9 | 45 | 100 | 45 | 100 | 46 | 97.83 | 45 | 100 | 45 | 100 | 51 | 88.24 | 10 | 46 | 100 | 46 | 100 | 46 | 100 | 47 | 97.87 | 48 | 95.83 | 50 | 92 | 11 | 109 | 100 | 116 | 93.97 | 143 | 76.22 | 208 | 52.40 | 463 | 23.54 | 600 | 18.16 |
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Residual activity = (activity of sample after incubation/activity of sample before incubation) × 100.
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