Research Article

BSA Nanoparticles for siRNA Delivery: Coating Effects on Nanoparticle Properties, Plasma Protein Adsorption, and In Vitro siRNA Delivery

Table 2

BSA NP plasma adsorption conditions and results as evaluated with immunoblots.

Fragment size (kDa)Fragment nameSystem
4.2 PLL24 PLL4.2 PEG-PLL24 PEG-PLL

Fibrinogen48 *** ** ** ***
56 *** ** *** ***
68 *** *** *** ***
<48Cleavage *0 * **
Albumin66 *** *** *** ***
C342Activation ** * ** **
70β * ** ** **
115α0000
Apolipoprotein A-1270+0+00
Plasminogen250000
600+*00

Plasma proteins eluted with 1.0 mL of 2% SDS in PBS; 0 indicates zero band intensity, while 0+ indicates trace band intensity and ***indicates highest intensity bands. Proteins shown in Table 1 but absent from this table were not observed in immunoblotting (zero band intensity throughout).