Stable Isotope-Resolved Metabolomic Differences between Hormone-Responsive and Triple-Negative Breast Cancer Cell Lines

Table 3

Fold-change differences for cell line intracellular (¹³C), consumption, and production (¹²C) concentration comparisons.

NMR signal	^13C Glc				^13C Gln				^12C
Cell lines	BT474	MCF-7	MDA-MD-231	MDA-MB-468	BT474	MCF-7	MDA-MD-231	MDA-MB-468	BT474	MCF-7	MDA-MB-231	MDA-MB-468
Metabolites	2-Hydroxy-butyrate
relative to BT474									1.00	-4.25	1.32	-0.50
relative to MCF-7									1.74	1.00	2.30	1.65
relative to MDA-MB-231									-2.43	-5.65	1.00	-2.81
relative to MDA-MB-468									1.05	-3.95	1.39	1.00
	Acetate
relative to BT474									1.00	-3.41	1.41	-1.99
relative to MCF-7									1.52	1.00	2.13	1.21
relative to MDA-MB-231									-2.89	-5.31	1.00	-4.31
relative to MDA-MB-468									1.25	-1.76	1.76	1.00
	Adenosine
relative to BT474	1.00	-8.77	-8.75	1.29	1.00	-9.80	-9.44	-1.04
relative to MCF-7	8.16	1.00	1.02	10.54	50.10	1.00	2.80	44.90
relative to MDA-MB-231	8.01	-0.17	1.00	10.36	17.89	-6.43	1.00	16.04
relative to MDA-MB-468	-2.26	-9.05	-9.03	1.00	1.12	-9.78	-9.38	1.00
	Alanine
relative to BT474	1.00	-8.65	-4.21	-4.59	1.00	-7.65	2.74	-5.42	1.00	-6.49	-4.25	-3.18
relative to MCF-7	7.43	1.00	4.31	4.02	4.25	1.00	11.65	1.95	2.85	1.00	1.64	1.94
relative to MDA-MB-231	1.73	-7.68	1.00	-0.66	-6.35	-9.14	1.00	-8.33	1.74	-3.89	1.00	1.19
relative to MDA-MB-468	1.85	-7.51	1.07	1.00	2.18	-4.86	5.98	1.00	1.47	-4.85	-1.57	1.00
	Aspartate
relative to BT474	1.00	-9.21	-1.08	-9.60	1.00	-9.09	1.04	-9.15
relative to MCF-7	12.62	1.00	11.25	-4.90	11.04	1.00	11.44	-0.63
relative to MDA-MB-231	1.12	-9.11	1.00	-9.55	-0.35	-9.13	1.00	-9.18
relative to MDA-MB-468	24.74	1.96	22.06	1.00	11.78	1.07	12.21	1.00
	AXP
relative to BT474	1.00	-7.32	-6.57	-0.47	1.00	23.30	35.50	54.20
relative to MCF-7	3.74	1.00	1.28	3.56	-9.57	1.00	1.52	2.33
relative to MDA-MB-231	2.91	-2.21	1.00	2.78	-9.72	-3.44	1.00	1.53
relative to MDA-MB-468	1.05	-7.19	-6.40	1.00	-9.82	-5.70	-3.45	1.00
	Glucose
relative to BT474	1.00	-6.90	-6.55	-5.60	1.00	2.17	1.44	-1.64	1.00	1.66	-1.19	1.16
relative to MCF-7	3.23	1.00	1.12	1.42	-5.38	1.00	-3.35	-0.47	-3.98	1.00	-4.69	-3.02
relative to MDA-MB-231	2.89	0.90	1.00	1.27	-3.05	1.50	1.00	-0.47	1.13	1.88	1.00	1.31
relative to MDA-MB-468	2.27	-2.96	-2.15	1.00	1.20	2.59	1.72	1.00	-1.37	1.43	-2.39	1.00
	Glutamate
relative to BT474	1.00	-4.58	-4.49	-4.64	1.00	-1.89	-5.21	-6.39	1.00	1.37	-7.16	-7.86
relative to MCF-7	1.85	1.00	1.02	-0.11	1.23	1.00	-4.09	-5.54	-2.71	1.00	-7.93	-8.44
relative to MDA-MB-231	1.82	-0.17	1.00	-0.28	2.09	1.69	1.00	-2.46	3.52	4.82	1.00	-2.48
relative to MDA-MB-468	1.87	1.01	1.03	1.00	2.77	2.24	1.33	1.00	4.68	6.42	1.33	1.00
	Glutamine
relative to BT474	1.00	-0.96	5.38	4.33	1.00	-6.06	-3.75	-7.30	1.00	1.14	1.46	1.34
relative to MCF-7	1.11	1.00	5.96	4.80	2.54	1.00	1.59	-3.13	-1.20	1.00	1.28	1.18
relative to MDA-MB-231	-8.14	-8.32	1.00	-1.95	1.60	-3.70	1.00	-5.67	-3.14	-2.20	1.00	-0.81
relative to MDA-MB-468	-7.69	-7.91	1.24	1.00	3.70	1.46	2.31	1.00	-2.53	-1.51	1.09	1.00
	Glutathione
relative to BT474	1.00	-9.47	-9.93	1.12	1.00	-9.20	-9.58	-7.35
relative to MCF-7	19.01	1.00	-8.72	21.27	12.53	1.00	-4.74	3.32
relative to MDA-MB-231	148.30	7.80	1.00	165.90	23.80	1.90	1.00	6.30
relative to MDA-MB-468	-1.06	-9.53	-9.94	1.00	3.78	-6.98	-8.41	1.00
	Glycine
relative to BT474	1.00	-9.99	-9.99	1.04	1.00	-8.92	-9.86	-7.99
relative to MCF-7	1098.10	1.00	1.00	1145.30	9.23	1.00	-8.72	1.86
relative to MDA-MB-231	1098.10	1.00	1.00	1145.30	72.00	7.80	1.00	14.50
relative to MDA-MB-468	-0.41	-9.99	-9.99	1.00	4.97	-4.62	-9.31	1.00
	Isoleucine
relative to BT474									1.00	-5.37	1.00	-2.25
relative to MCF-7									2.16	1.00	2.17	1.67
relative to MDA-MB-231									1.00	-5.38	1.00	-2.28
relative to MDA-MB-468									1.29	-4.02	1.29	1.00
	Lactate
relative to BT474	1.00	-2.51	-7.82	-2.99	1.00	1.98	-2.07	1.12	1.00	2.08	-1.46	1.21
relative to MCF-7	1.33	1.00	-7.09	-0.65	-4.95	1.00	-5.99	-4.35	-5.18	1.00	-5.89	-4.17
relative to MDA-MB-231	4.58	3.43	1.00	3.21	1.26	2.50	1.00	1.41	1.17	2.43	1.00	1.42
relative to MDA-MB-468	1.43	1.07	-6.89	1.00	-1.06	1.77	-2.91	1.00	-1.73	1.72	-2.94	1.00
	Leucine
relative to BT474									1.00	-2.93	-1.89	-2.16
relative to MCF-7									1.41	1.00	1.15	1.11
relative to MDA-MB-231									1.23	-1.28	1.00	-0.33
relative to MDA-MB-468									1.28	-0.99	1.03	1.00
	Methionine
relative to BT474									1.00	-4.73	-2.32	-2.37
relative to MCF-7									1.90	1.00	1.46	1.45
relative to MDA-MB-231									1.30	-3.14	1.00	-0.07
relative to MDA-MB-468									1.31	-3.09	1.01	1.00
	Phenylalanine
relative to BT474									1.00	-1.36	2.85	3.35
relative to MCF-7									1.16	1.00	3.29	3.88
relative to MDA-MB-231									-6.49	-6.96	1.00	1.18
relative to MDA-MB-468									-7.02	-7.43	-1.52	1.00
	Proline
relative to BT474	1.00	4.05	2.29	2.78	1.00	4.53	1.48	1.49
relative to MCF-7	-7.53	1.00	-4.34	-3.15	-7.79	1.00	-6.72	-6.71
relative to MDA-MB-231	-5.64	1.77	1.00	1.21	-3.26	3.05	1.00	1.00
relative to MDA-MB-468	-6.40	1.46	-1.74	1.00	-3.29	3.04	1.00	1.00
	Threonine
relative to BT474									1.00	1.41	2.77	3.36
relative to MCF-7									-2.90	1.00	1.97	2.39
relative to MDA-MB-231									-6.39	-4.91	1.00	1.21
relative to MDA-MB-468									-7.03	-5.81	-1.76	1.00
	Tryptophan
relative to BT474									1.00	1.04	1.57	1.30
relative to MCF-7									-0.36	1.00	1.51	1.25
relative to MDA-MB-231									-3.63	-3.39	1.00	-1.74
relative to MDA-MB-468									-2.29	-2.00	1.21	1.00
	Tyrosine
relative to BT474									1.00	1.18	1.31	1.36
relative to MCF-7									-1.51	1.00	1.11	1.15
relative to MDA-MB-231									-2.38	-1.03	1.00	1.03
relative to MDA-MB-468									-2.64	-1.33	-0.33	1.00
	UDP-GlcNAc
relative to BT474	1.00	-9.17	-4.18	-7.05	1.00	-5.65	-8.34	-8.19
relative to MCF-7	12.12	1.00	7.05	3.57	2.30	1.00	-6.19	-5.84
relative to MDA-MB-231	1.72	-8.58	1.00	-4.94	6.03	2.62	1.00	1.09
relative to MDA-MB-468	3.39	-7.20	1.98	1.00	5.53	2.41	-0.83	1.00
	UDP-Glucose
relative to BT474	1.00	-7.49	-4.94	-5.14	1.00	-0.41	1.97	3.75
relative to MCF-7	3.98	1.00	2.01	1.93	1.04	1.00	2.06	3.91
relative to MDA-MB-231	1.98	-5.03	1.00	-0.40	-4.93	-5.14	1.00	1.90
relative to MDA-MB-468	2.06	-4.83	1.04	1.00	-7.33	-7.44	-4.74	1.00
	Uridine
relative to BT474	1.00	19.04	5.21	14.75	1.00	-6.85	1.80	1.74
relative to MCF-7	-9.47	1.00	-7.26	-2.26	3.17	1.00	5.71	5.52
relative to MDA-MB-231	-8.08	3.65	1.00	2.83	-4.44	-8.25	1.00	-0.33
relative to MDA-MB-468	-9.32	1.29	-6.46	1.00	-4.25	-8.19	1.03	1.00
	Uridine/UDP-G
relative to BT474	1.00	-6.83	-4.15	-4.52	1.00	-9.88	-8.88	-6.21
relative to MCF-7	3.15	1.00	1.84	1.73	86.50	1.00	9.70	32.80
relative to MDA-MB-231	1.71	-4.58	1.00	-0.63	8.92	-8.97	1.00	3.38
relative to MDA-MB-468	1.82	-4.21	1.07	1.00	2.64	-9.70	-7.04	1.00
	Valine
relative to BT474									1.00	-4.12	-0.75	-2.48
relative to MCF-7									1.70	1.00	1.57	1.28
relative to MDA-MB-231									1.08	-3.65	1.00	-1.88
relative to MDA-MB-468									1.33	-2.18	1.23	1.00

Fold-change differences highlighted are magnitude ≥ ±2 for ¹³C intracellular (Table 1) and ¹²C production and consumption concentration (Table 2) comparisons relative to each cell line and between them. Concentration values of zero in Tables 1 and 2 were set to 1.00 for fold-change calculations. All fold-change differences less than ≥ 2-fold are in italics.