Stable Isotope-Resolved Metabolomic Differences between Hormone-Responsive and Triple-Negative Breast Cancer Cell Lines
Table 2
Metabolite consumption and production in conditioned medias.
Metabolite
BT474
MCF-7
MDA-MB-231
MDA-MB-468
Glucose
30381.9 (4849.4)
50449.5 (2418.9)
26775.3 (14543.3)
35193.2 (10767.5)
Glutamine
2520.4 (1605.3)
2865.5 (487.3)
3673.5 (1042.1)
3376.1 (746)
Isoleucine
1088.1 (194.2)
504.3 (156.5)
1091.9 (94.3)
843.4 (142.3)
Leucine
1897.8 (392.2)
1341.3 (197.9)
1538.6 (244.2)
1488.2 (126.3)
Methionine
330.5 (102.2)
174.2 (160.1)
253.9 (81.2)
252.2 (38.8)
Phenylalanine
55.0 (52.9)
47.5 (47.2)
156.5 (159.2)
184.5 (80.1)
Threonine
133.1 (133.3)
187.5 (163.7)
368.6 (324.3)
447.5 (140.6)
Tryptophan
31.9 (35.3)
33.1 (29.3)
50.1 (22.9)
41.4 (16.6)
Tyrosine
158.6 (95.6)
186.8 (52.2)
208.2 (122.5)
215.4 (87.4)
Valine
1141.9 (250.6)
671.5 (162.1)
1056.7 (124.9)
858.3 (16)
2-Hydroxy-butyrate
388.4 (173.5)
223.3 (51.2)
513.1 (150.9)
368.8 (172.5)
Acetate
144.4 (67.4)
95.2 (30)
203.2 (124.5)
115.6 (124.6)
Alanine
3420.3 (978.3)
1200.9 (29.8)
1966.6 (498.4)
2333.5 (332.8)
Glutamate
1010.2 (390.5)
1385.2 (306.5)
287.1 (124.8)
215.9 (302.8)
Lactate
56700 (7688)
117705.8 (5518)
48395.6 (27755.1)
68584.2 (23542.9)
Averages (in nmol/mg) calculated from 12C signals. Standard deviations in parentheses normalized to dried pellet weight. Consumption is reflected by bolded metabolites. Production is reflected by italicized metabolites.
