Stable Isotope-Resolved Metabolomic Differences between Hormone-Responsive and Triple-Negative Breast Cancer Cell Lines
Table 1
Semiquantitative concentrations of intracellular metabolites.
BT474
MCF-7
MDA-MD-231
MDA-MB-468
Metabolites
13C Glc
13C Gln
13C Glc
13C Gln
13C Glc
13C Gln
13C Glc
13C Gln
Adenosine
229.2 (210.1)
50.1 (60.4)
28.1 (35)
0 (0)
28.6 (32)
2.8 (4.8)
296.2 (39.7)
44.9 (17.2)
Alanine
7293.5 (2003)
56.5 (97.9)
981.1 (191.1)
13.3 (23.1)
4225.2 (389.6)
155 (158.4)
3947.3 (468.7)
25.9 (26.8)
Aspartate
762.1 (236.5)
526.4 (63.8)
60.4 (104.6)
47.7 (50.9)
679.5 (133)
545.7 (101.3)
30.8 (31.5)
44.7 (39.9)
AXP
278.4 (171.9)
0 (0)
74.5 (50.7)
23.3 (10.6)
95.6 (22.4)
35.5 (27.9)
265.4 (62.9)
54.2 (36.8)
Glucose
6791.1 (2295.2)
422.3 (327)
2103.3 (128.9)
914.6 (440.3)
2346 (168.3)
607.9 (96.1)
2989.7 (743.3)
353 (83.1)
Glutamate
1765.2 (531.5)
1103.6 (242.6)
956 (227.8)
894.6 (88.5)
972.3 (163.2)
528.8 (120.7)
945.3 (205.8)
398.9 (145.5)
Glutamine
534.9 (497.9)
7697.9 (1913.5)
483.4 (285.1)
3030 (128.1)
2879.8 (512)
4812.5 (1445.6)
2318 (474.2)
2081.8 (667.6)
Glycine
1098.1 (374.1)
72 (124.7)
0 (0)
7.8 (13.6)
0 (0)
0 (0)
1145.3 (169.4)
14.5 (25.2)
Lactate
2167.3 (653.3)
75.4 (15.1)
1623.7 (515.1)
149.3 (26.9)
472.8 (63.5)
59.8 (32.7)
1518.7 (320.8)
84.3 (51)
Glutathione
148.3 (72.4)
23.8 (41.3)
7.8 (13.5)
1.9 (3.4)
0 (0)
0 (0)
165.9 (67.7)
6.3 (11)
Proline
170.2 (131.3)
108.5 (86.9)
689.4 (183.5)
491 (198)
390.5 (128.8)
160.9 (45.4)
472.5 (68.1)
161.6 (14.7)
UDP-GlcNAc
1212.9 (387.9)
146 (127.7)
100.1 (86.8)
63.5 (12.5)
705.9 (126.7)
24.2 (4.6)
357.4 (107.1)
26.4 (4)
UDP-Glucose
1422.4 (267.1)
21.9 (38)
357.2 (79.9)
21 (18.4)
719.4 (80.6)
43.2 (68.4)
690.6 (93)
82.1 (16.5)
Uridine
15.9 (27.5)
18.4 (31.9)
302.8 (46)
5.8 (10)
82.9 (71.4)
33.1 (27.8)
234.5 (37.9)
32 (46.2)
Uridine/UDP-G
2275.9 (409.5)
86.5 (149.8)
721.7 (122.3)
0 (0)
1331.4 (127.8)
9.7 (16.9)
1247.3 (310.5)
32.8 (38.6)
Semiquantitative values have been normalized to pellet weight and are unitless. Values represent average among three replicates and the standard deviation is in parentheses.
