Synthesis, Structural Characterization, and Preclinical Efficacy of a Novel Paclitaxel-Loaded Alginate Nanoparticle for Breast Cancer Treatment

Figure 6

DNA content and cell cycle analysis of (a) control (untreated) breast cancer patient primary tumor cells, (b) breast cancer patient primary tumor cells treated with PTX for 24 h, and (c) breast cancer patient primary tumor cells treated with PTX-ALG nanoparticles for 24 h. M1 gate indicates the G0-G1 phase of diploid cells, M2 gate indicates the G0-G1 phase for the hyperdiploid cells, M3 gate indicates the S phase and M4 gate indicates the G2-M phase for the hyperdiploid cells, and M5 gate indicates the sub-G0-G1 (apoptotic) population. The S and G2-M phases of diploid cells were masked by the phases of hyperdiploid cells. All analysis of cell cycle was after gating of living cells to exclude most apoptotic cells.

(a)

(b)

(c)