Alzheimer’s Disease Frontal Cortex Mitochondria Show a Loss of Individual Respiratory Proteins but Preservation of Respiratory Supercomplexes

Figure 5

Relationships among levels of VDAC-normalized OXPHOS subunits in frontal cortex compared to their gene expression values from Cufflinks analysis of RNAseq data. Total RNA was extracted from AD and CTL frontal cortex samples, subjected to multiplex paired-end RNA sequencing and gene expressions analyzed as described in Methods. These expression levels (as FPKM) were plotted against relative levels of their respective respiratory proteins as assayed by Western blotting of mitochondrial preparations from the same samples (see Methods for details).