Alzheimer’s Disease Frontal Cortex Mitochondria Show a Loss of Individual Respiratory Proteins but Preservation of Respiratory Supercomplexes

Figure 3

AD mitochondrial preparations from frontal cortex show reductions in all OXPHOS subunits representative of complexes I-V. Shown are immunoblot results for mitochondrial preparations of frontal cortex from CTL and AD samples, extracted with RIPA buffer, electrophoresed and immunoblotted as described in Methods, and normalized to mitochondrial mass (VDAC). All OXPHOS subunits showed significant reductions in AD mitochondria (2-way ANOVA), and several subunits showed individual declines in AD brain (t-test). (top) OXPHOS subunit levels are expressed as % mean CTL levels. (bottom) OXPHOS subunit raw data. Abbreviations as in Figure 2.