Amyloid-Beta 1-42 Cross-Reactive Antibody Prevalent in Human Sera May Contribute to Intraneuronal Deposition of A-Beta-P-42

Figure 1

Reaction of monoclonal antibody to Aβ42 with Aβ42 peptide and different proteins directly or indirectly involved with AD. The mean ± 3SD of 12 determinations for each antigen is shown. Compared to the monoclonal antibody’s reaction with amyloid-β-42 as positive control and HSA or unimmunized rabbit serum as negative control, the reaction of this antibody with ATP-synthase, claudin-5, and asialoganglioside GM₁ is nonreactive, with S100-B, GFAP, and MBP is low or weakly positive, with α-synuclein and AQP4 is moderate, with tau protein and rabaptin-5 is highly positive, and with Aβ protein and presenilin is very highly positive. 0-0.27 OD: nonreactive, 0.271-0.50: equivocal, 0.51-1.2: low positive, 1.21-2.0: moderately positive, 2.01-3.0: highly positive, and >3.0: very highly positive.