Centella asiatica Extract Improves Behavioral Deficits in a Mouse Model of Alzheimer's Disease: Investigation of a Possible Mechanism of Action
Figure 6
HPLC comparison of CA water and ethanol extracts made from the same batch of plant material. Asiatic acid (AA) and madecassic acid (MA) are detected in the ethanolic extract, but not in the water extract (GKW). The water extract GKW contains mostly very polar compounds as shown by their earlier elution than the triterpenes AA and MA. HPLC was conducted on an Aquasil C18 column with acetonitrile:water gradient with 0.1% acetic acid. Acetonitrile concentration 10% at 0 min, 50% at 25 min, 90% at 40 min, then returning to starting conditions, detector wavelength 205 nm. AA and MA were identified by comparison of retention times to those of commercial standards.
