Research Article
Determination of Crizotinib in Mouse Tissues by LC-MS/MS and Its Application to a Tissue Distribution Study
Table 4
Stability data of crizotinib in mouse liver and small intestines under various storage conditions (n = 3).
| Storage conditions | Added | Liver | Small intestine | (ng/mL) | Measured (ng/mL) | RE (%) | Measured (ng/mL) | RE (%) |
| Room temperature for 8 h | 60 | 58.38 ± 7.50 | −2.7 | 58.71 ± 8.59 | −2.1 | 6400 | 6520.6 ± 137.9 | 1.9 | 5887.3 ± 107.9 | −8.0 | Autosampler for 10 h (4°C) | 60 | 62.05 ± 3.74 | 3.4 | 64.49 ± 4.06 | 7.5 | 6400 | 6844.3 ± 79.3 | 6.9 | 6682.8 ± 348.0 | 4.4 | Three freeze-thaw cycles | 60 | 60.10 ± 3.35 | 0.2 | 64.21 ± 0.89 | 7.0 | 6400 | 6978.5 ± 291.4 | 9.0 | 6833.4 ± 92.78 | 6.8 | Freezing for 30 days (−40°C) | 60 | 58.97 ± 7.04 | −1.7 | 57.70 ± 1.24 | −3.8 | 6400 | 5803.0 ± 148.3 | −9.3 | 6758.7 ± 209.8 | 5.6 |
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RSD, relative standard deviation; RE, relative error; n, number of replicates.
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