Genome Editing in Plants: An Overview of Tools and Applications

Table 2

Examples of application of genome editing technology in plants.

Number	Purpose or traits studied	Plant species	Method used	References	Year
1	Gene modification	Arabidopsis thaliana	ZFN	Osakabe et al. [15]	2010
2	Gene modification	Arabidopsis thaliana	ZFN	Zhang et al. [16]	2010
3	Gene editing	Nicotiana tabacum	ZFN	Townsend et al. [17]	2009
4	Targeted integration into endogenous loci	Zea mays	ZFN	Shukla [18]	2009
5	Targeted mutagenesis	Arabidopsis thaliana	TALEN	Christian et al. [19]	2013
6	Targeted mutagenesis	Oryza sativa	TALEN	Zhang et al. [20]	2016
7	Gene editing	Oryza sativa	TALEN	Li et al. [21]	2012
8	Targeted transcriptional gene repression	Arabidopsis thaliana	TALEN	Mahfouz et al. [22]	2012
9	Herbicide resistance	Arabidopsis thaliana	ODM	Sauer et al. [7]	2016
10	Herbicide resistance	Nicotiana tabacum	ODM	Sauer et al. [7]	2016
11	Herbicide resistance	Oryza sativa	ODM	Sauer et al. [7]	2016
12	Herbicide resistance	Zea mays	ODM	Sauer et al. [7]	2016
13	Herbicide resistance	Brassica napus	ODM	Sauer et al. [7]	2016
14	Gene knockout or editing	Arabidopsis thaliana	CRISPR/Cas	Li et al. [8]	2013
				Mao et al. [13]	2013
15	Gene knockout or editing	Nicotiana benthamiana	CRISPR/Cas	Li et al. [8]	2013
16	Gene knockout or editing	Nicotiana tabacum	CRISPR/Cas	Gao et al. [23]	2015
17	Gene knockout or editing	Oryza sativa	CRISPR/Cas	Mao et al. [13]	2013
18	Multiplex genome editing	Arabidopsis thaliana	CRISPR/Cas	Li et al. [8]	2013
				Mao et al. [13]	2013
19	Multiplex genome editing	Nicotiana tabacum	CRISPR/Cas	Cong et al. [24]	2013
				Gao et al. [23]	2015
20	Gene insertion or replacement	Arabidopsis thaliana	CRISPR/Cas	Mao et al. [13]	2013
21	Gene insertion or replacement	Nicotiana benthamiana	CRISPR/Cas	Li et al. [8]	2013
22	Epigenetic gene regulation	Arabidopsis thaliana	CRISPR/Cas	Puchta [25]	2016
23	Targeted mutagenesis	Glycine max	CRISPR/Cas	Jacobs et al. [26]	2015
24	Targeted mutagenesis	Oryza sativa	CRISPR/Cas	Xu et al. [27]	2016
25	Improvement of nutritional value	Camelina sativa	CRISPR/Cas	Jiang et al. [28]	2017
26	Yield improvement	Solanum lycopersicum	CRISPR/Cas	Soyk et al. [29]	2017
27	Yield improvement	Zea mays	CRISPR/Cas	Peng et al. [30]	1999
28	Salt resistance	Oryza sativa	CRISPR/Cas	Osakabe et al. [31]	2016
29	Resistance to drought	Oryza sativa	CRISPR/Cas	Shan et al. [32]	2013
30	Herbicide resistance	Zea mays	CRISPR/Cas	Svitashev et al. [33]	2015
31	Biotic stress	Triticum aestivum	CRISPR/Cas	Wang et al. [34]	2014
32	Biotic stress	Oryza sativa	CRISPR/Cas	Li et al. [21]	2012
33	Biotic stress	Arabidopsis thaliana	CRISPR/Cas	Ji et al. [35]	2015
		Nicotiana benthamiana		Ali et al. [36]	2015
34	Biotic stress	Nicotiana benthamiana	CRISPR/Cas	Baltes et al. [37]	2015
35	Biotic stress	Oryza sativa	CRISPR/Cas	Liu et al. [38]	2012
36	Biotic stress	Oryza sativa	CRISPR/Cas	Wang et al. [39]	2016
37	Biotic stress	Cucumis sativus	CRISPR/Cas	Chandrasekaran et al. [40]	2016