Tetracycline Effects on Candida Albicans Virulence Factors

Figure 4

Biofilm was produced in the YNB growth media with or without the addition of tetracycline (0.1 𝜇g/mL). Biofilm was measured by allowing the metabolic indicator Alimar Blue to be reduced by the biomass remaining after vigorous washing of the wells. The relative biofilm measure represents the dye reduction over a 2-hour period measured at 600 nm spectrophotometrically. Of note, 5 of the 6 strains tested showed a tendency toward increased biofilm metabolic activity when exposed to tetracycline, though the variability of the biological assay limited definite conclusions regarding excess biofilm production.