Downregulation of LncRNA SNHG7 Sensitizes Colorectal Cancer Cells to Resist Anlotinib by Regulating miR-181a-5p/GATA6

Figure 4

SNHG7 promotes GATA6 expression via sponging miR-181a-5p. (a) Potential binding site of miR-181a-5p in GATA6 found by TargetScan. (b) Luciferase reporter assay to verify the interaction between miR-181a-5p and GATA6 in HCT116/ATB and LOVO/ATB cell lines. miR-181a-5p overexpression inhibited Luciferase activity, but the inhibition disappeared when the binding site was mutated in GATA6. (c) Western blot and qRT-PCR showing the downregulation of GATA6 by SNHG7 knockdown and the upregulation of GATA6 by the transfection of miR-181a-5p in HCT116/ATB and LOVO/ATB cell lines. (d) qRT-PCR measurement to check the expression level of GATA6 in 50 pairs of CRCs and the adjacent tissues. A significant negative correlation between GATA6 and miR-181a-5p and a significant positive correlation between GATA6 and SNHG7 were observed by Spearman correlation analysis in 50 cases of CRC. The statistical differences were evaluated by Student’s t-test (*, **, and ***).

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