hsa_circRNA_000166 Facilitated Cell Growth and Limited Apoptosis through Targeting miR-326/LASP1 Axis in Colorectal Cancer

Figure 3

Decreased hsa_circRNA_000166 expression affected cell growth and apoptosis in CRC cells. (a) The transcriptional level of hsa_circRNA_000166 was measured by qRT-PCR in both HCT116 and SW480 cells after transfection with siRNAs. (b) Cell proliferation assay showed that decreased hsa_circRNA_000166 expression limited the growth of HCT116 and SW480 cells after siRNA-1 or siRNA-2 transfection compared with the controls. (c) Clone formation assay demonstrated that the number of colonies was significantly decreased after siRNA-1 or siRNA-2 transfection compared with the controls in HCT116 and SW480 cells. (d) Flow cytometric analysis displayed that the number of apoptotic cells was noticeably increased in siRNA-1- or siRNA-2-transfected groups compared with the controls. The Student -test was used for statistics.

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