Helicobacter pylori CagA and IL-1β Promote the Epithelial-to-Mesenchymal Transition in a Nontransformed Epithelial Cell Model

Figure 3

Nuclear translocation of β-catenin correlates with increased expression of ZEB1 and Snail1. (a) Immunoblotting of whole cell lysates of infected or IL-1β-stimulated cells with anti-β-catenin and anti-E-cadherin antibodies. The relative expression of (b) Snail1 and CDH1 and (c) ZEB1 genes was determined by semiquantitative RT-PCR. Expression of housekeeping genes RPLP0 and GAPDH was used as internal control. Figures are representative of three independent experiments performed in duplicate or triplicate. Figures are representative of three independent experiments performed in duplicate or triplicate.

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