Insight into the Mechanistic Basis of the Hysteretic-Like Kinetic Behavior of Thioredoxin-Glutathione Reductase (TGR)

Figure 8

Effect of GSSG on the reduction of human Trx by T. crassiceps TGR. Reaction mixtures were prepared in buffer A by mixing Trx with 100 μM NADPH either in the presence or in the absence of 2 mM GSSG. After two minutes, enzyme was added to start the reaction. The final volume of the reaction mixture was 120 μL. (Δ) 2 mM GSSG, no trx; (●) 150 μM Trx, no GSSG; (▲) 2 mM GSSG plus 60 μM Trx; (○) 2 mM GSSG plus 150 μM Trx. The final concentration of enzyme was 37 nM.