Agroindustrial Wastes as Alternative for Lipase Production by Candida viswanathii under Solid-State Cultivation: Purification, Biochemical Properties, and Its Potential for Poultry Fat Hydrolysis
Table 6
Effect of organic solvents on crude lipase stability produced by C. viswanathii under solid-state cultivation.
Organic solvent
Crude enzyme
Purified enzyme
Relative activity (%)
Stability (%)
Relative activity (%)
Stability (%)
Control
—
100.0 ± 1.2
100.0 ± 2.0
100.0 ± 2.4
100.0 ± 3.2
Glycerol
−1.67
135.4 ± 4.2
99.5 ± 2.6
109.4 ± 5.7
60.2 ± 2.0
DMSO
−1.38
111.7 ± 4.0
99.8 ± 3.0
113.5 ± 2.5
59.5 ± 3.4
Propylene glycol
−0.92
111.5 ± 3.1
88.7 ± 3.5
97.5 ± 7.7
60.1 ± 3.4
Methanol
−0.76
105.1 ± 3.3
84.6 ± 1.9
103.2 ± 7.5
52.9 ± 6.1
Acetonitrile
−0.40
86.2 ± 1.3
68.1 ± 3.9
88.6 ± 8.2
58.6 ± 2.8
Ethanol
−0.24
106.2 ± 2.2
90.5 ± 4.8
106.8 ± 6.1
94.5 ± 2.1
Acetone
−0.23
90.5 ± 2.2
66.7 ± 2.4
85.8 ± 6.8
69.2 ± 1.8
1-Propanol
0.07
97.9 ± 2.5
72.4 ± 1.3
111.5 ± 9.9
66.9 ± 1.8
2-Propanol
0.25
78.3 ± 2.6
56.0 ± 2.4
83.0 ± 4.3
59.2 ± 1.4
n-Butanol
0.80
92.0 ± 4.0
12.1 ± 2.6
84.1 ± 9.9
58.3 ± 3.5
Toluene
2.50
91.1 ± 3.3
69.8 ± 1.9
87.2 ± 5.3
25.0 ± 2.6
Xylol
3.15
65.9 ± 1.4
51.5 ± 1.3
82.0 ± 7.4
39.1 ± 4.2
n-Hexane
3.50
105.9 ± 2.5
98.0 ± 4.7
98.5 ± 8.6
65.2 ± 3.1
Isooctane
4.51
90.4 ± 2.4
95.1 ± 3.4
91.4 ± 7.8
61.8 ± 2.0
Assay conditions: for stability assays crude and purified lipase were incubated at 30°C at 200 rpm during 2 h and the activity was assayed with p-NPP using McIlvaine buffer pH 5.0, at 50°C. : logarithm of the partition coefficient () in octanol/water two-phase system indicates the solvents hydrophobicity. DMSO: dimethyl sulfoxide.
