Agroindustrial Wastes as Alternative for Lipase Production by Candida viswanathii under Solid-State Cultivation: Purification, Biochemical Properties, and Its Potential for Poultry Fat Hydrolysis

Table 3

Effect of temperature and moisture on lipase production by C. viswanathii in solid-state cultivation.

Parameter	Lipase activity (U/gds)	Specific activity (U/mg prot)
Temperature (°C)
15	26.10 ± 4.42	13.31 ± 1.92
20	56.05 ± 6.07	22.97 ± 2.43
25	77.71 ± 3.36	28.59 ± 2.71
30	143.36 ± 9.65	65.76 ± 3.18
35	129.11 ± 8.15	43.19 ± 2.98
40	25.80 ± 6.17	10.05 ± 1.56
Moisture (%)
20	98.70 ± 4.87	78.89 ± 3.47
30	147.55 ± 8.43	111.32 ± 4.55
40	157.33 ± 9.25	136.20 ± 5.74
50	143.36 ± 7.47	113.32 ± 6.40
60	137.56 ± 6.34	74.56 ± 5.70
70	92.43 ± 5.78	60.80 ± 4.85

Cultures were carried out using wheat bran plus barley spent grain (1 : 1, w/w), 40% (w/w) poultry fat, and 3.5% (w/w) yeast extract for 5 days (above). Cultures were carried out in the same conditions and at 30°C (below).