Purification and Characterization of Phenylalanine Ammonia Lyase from Trichosporon cutaneum

Table 3

Kinetic parameters for purified extracts of the PAL enzyme from Trichosporon cutaneum.

Trial	Phenylalanine	Phenylalanine	Phenylalanine	Tyrosine	Tyrosine	Tyrosine	PAL/TAL ratio
	𝑉max (𝜇M min^−1)	𝐾𝑚 (mM)	𝑉max/𝐾𝑚	𝑉max (𝜇M min^−1)	𝐾^′ (mM)	𝑉max/𝐾^′
1	1.3±0.1	5.0±1.1	0.3	0.2±0.02	2.4±0.6	0.1	3.0
2	4.7±0.6	5.7±3.0	0.8	1.0±0.1	2.0±0.4	0.5	1.6
3	7.2±0.8	4.2±2.0	1.7	0.8±0.1	0.9±0.4	0.9	1.9

For each trial, activity assays for Phe and Tyr were performed on the same enzyme extract. Extracts were obtained from anion exchange chromatography.