Kinetic Analysis of Guanidine Hydrochloride Inactivation of β-Galactosidase in the Presence of Galactose
Scheme 1
, , , , , and represent the native enzyme, denatured enzyme, the Michaelis constant, the turnover number of enzyme catalysed reaction in the presence of denaturant, the first order microscopic rate constant for the free enzyme, and the first order microscopic rate constant for the enzyme-substrate complex, respectively. All the kinetic constants are functions of the denaturant concentrations and thus functions of the apparent inactivation rate constant, .
