Kinetic Analysis of Guanidine Hydrochloride Inactivation of β-Galactosidase in the Presence of Galactose

Figure 5

Plots of [𝑃]_∞ against [𝑆] for β-galactosidase in the presence of 3 M GdnHCl—5 mM galactose, 3 M GdnHCl—10 mM galactose, and 3 M GdnHCl—20 mM galactose. Inset is the magnification of the graph of [𝑃]_∞ versus [𝑆] for 3 M GdnHCl—5 mM galactose showing that the intercept on the 𝑦-axis is slightly above the zero point.