Kinetic Analysis of Guanidine Hydrochloride Inactivation of β-Galactosidase in the Presence of Galactose

Figure 3

Semilogarithmic plot of P(μM) versus time (𝑡) of data: (a) ln([𝑃]_∞ − [𝑃]_𝑡) versus 𝑡 for 3 M GdnHCl, 0 mM galactose; (b) 3 M GdnHCl, 5 mM galactose; (c) 3 M GdnHCl, 10 mM galactose; (d) 3 M GdnHCl, 20 mM galactose. N.B: [𝑃]_∞ = [pNP]; [𝑃]_𝑡 = [pNP]𝑡.

(a)

(b)

(c)

(d)