Research Article

Kinetic Analysis of Guanidine Hydrochloride Inactivation of β-Galactosidase in the Presence of Galactose

Figure 2

Effect of GdnHCl and galactose on the 𝐾 𝑚 and 𝑉 m a x of β-galactosidase using pNPG as substrate, both in the presence and absence of galactose. 𝐾 𝑚 and 𝑉 m a x values were calculated from Lineweaver-Burk plots of initial velocity data at the concentrations of GdnHCl and galactose indicated.
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