Research Article
Improved Laccase Production by Trametes pubescens MB89 in Distillery Wastewaters
Table 2
Laccase synthesis obtained with the addition of various reported inducers prior to inoculation or four days thereafter (
).
| Reported inducer | Added prior to inoculation | Added after four days | HLA* (units/L) | Day of HLA | Increase (fold) | HLA (units/L) | Day of HLA | Increase (fold) |
| 2,5-Xylidine | 8419 | 2 | 3.7 | 2944 | 11 | 2.4 | Ethanol | 6701 | 20 | 2.9 | 292 | 6 | 0.2 | Copper | 5492 | 20 | 2.4 | 1044 | 13 | 0.9 | 4-Methylcatechol | 1153 | 20 | 0.5 | 2253 | 13 | 1.9 | n-hydroxyphthalimide | 1636 | 14 | 0.7 | 2283 | 13 | 1.9 | Gallic acid | 3303 | 14 | 1.4 | 820 | 8 | 0.7 | Tannic acid | 3114 | 20 | 1.4 | 1111 | 13 | 0.9 | Quercetin | 2966 | 14 | 1.3 | 588 | 13 | 0.5 | Syringic acid | 1862 | 14 | 0.8 | 1404 | 11 | 1.2 | Guaiacol | 2580 | 16 | 1.1 | 1292 | 11 | 1.1 | Dimethoxybenzyl alcohol | 1939 | 16 | 0.8 | 1247 | 13 | 1.0 | Phenol | 2149 | 14 | 0.9 | 1270 | 11 | 1.0 | Violuric acid | 2035 | 14 | 0.9 | 1039 | 11 | 0.9 | Phenol red | 2601 | 12 | 1.1 | 663 | 13 | 0.5 | Cellulose | 2474 | 16 | 1.1 | 328 | 13 | 0.3 | p-Coumaric acid | 2370 | 14 | 1.0 | 1227 | 13 | 1.0 | Rooibos | 2119 | 16 | 0.9 | 372 | 13 | 0.3 | o-Cresol | 2064 | 12 | 0.9 | 558 | 11 | 0.5 | Dichloroindophenol | 127 | 12 | 0.1 | 795 | 8 | 0.7 | Hydroxybenzotriazole | 977 | 14 | 0.4 | 656 | 13 | 0.5 | Cycloheximide | 20 | 4 | 0.0 | 455 | 5 | 0.4 | Control | 2305 | 12 | 1.0 | 1214 | 13 | 1.0 |
|
|
*HLA: highest laccase activity.
|